Antispasmodic Effects of Some N-substituted 2- Methyl Imidazole Derivatives on Guinea Pig Ileum

A series of novel N-substituted 2-methyl imidazole derivatives have been synthesized, and their in vitro antispasmodic activity was assessed on contractions of isolated guinea pig ileum, induced by acetylcholine (5× 10M to 1× 10M), and compared with the effect of atropine. All the prototypes were synthesized and confirmed by their FTIR, HNMR, MASS and elemental spectral data. Antispasmodic activity of all prototypes were tested by bioassay at various concentrations (10, 50 and 100 μg/ml), and concentration-response curves were plotted to check their ability to reverse the activity of acetylcholine on prior contact with the ileum. All the compounds 1(a-d) were producing a competitive antagonistic action at (10 μg/ml), and at higher concentrations (50 and 100 μg/ml) the curves shifted to the right showing significant antagonism (P<0.05, P<0.01 and P<0.001) which is probably mediated through muscarinic receptors.